Optimization of polyethylenimine-mediated transient transfection using response surface methodology design
نویسندگان
چکیده
منابع مشابه
Optimization of lentiviral vector production using polyethylenimine-mediated transfection
The aim of the present study was to optimize the polyethylenimine (PEI)-mediated transfection method in order to simplify the efficient production of lentiviral vectors (LvVs), and to compare the CaPO4- and PEI-mediated transfection methods for producing LvVs. Different titration methods of LvV stocks, as well as different culture media, culture durations, cell densities and DNA quantities were...
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Since Amaranth (AM) is one of the dye compounds which is harmful to human’s life its removal from industrial waste water would reduce their environmental impact and health effect. Copper nanoparticle (CuNP) is a simple and eco-friendly material which can be used to remove this pollutant. In this paper, copper nanoparticles were synthesized, for removal of AM dye. The experiments were designed b...
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A soil bacterium capable of degrading chitin on chitin agar plates was isolated and identified as Bacillus pumilus isolate U5 on the basis of 16S rDNA sequence analysis. In order to optimize culture conditions for chitinase production by this bacterium, a two step approach was employed. First, the effects of several medium components were studied using the Plackett-Burman design. Among various ...
متن کاملOptimization of Chitinase Production by Bacillus pumilus Using Plackett-Burman Design and Response Surface Methodology
A soil bacterium capable of degrading chitin on chitin agar plates was isolated and identified as Bacillus pumilus isolate U5 on the basis of 16S rDNA sequence analysis. In order to optimize culture conditions for chitinase production by this bacterium, a two step approach was employed. First, the effects of several medium components were studied using the Plackett-Burman design. Among various ...
متن کاملoptimization of chitinase production by bacillus pumilus using plackett-burman design and response surface methodology
a soil bacterium capable of degrading chitin on chitin agar plates was isolated and identified as bacillus pumilus isolate u5 on the basis of 16s rdna sequence analysis. in order to optimize culture conditions for chitinase production by this bacterium, a two step approach was employed. first, the effects of several medium components were studied using the plackett-burman design. among various ...
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ژورنال
عنوان ژورنال: Electronic Journal of Biotechnology
سال: 2010
ISSN: 0717-3458,0717-3458
DOI: 10.2225/vol13-issue5-fulltext-6